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Until the end of the study the Edgerton Laboratory was blinded as to the identity of the irradiated rats, i.e. the rats were shipped as groups A and B. Upon arrival, each of the rats was housed in individual large cage, and bladder expressions were performed 2 3 times per day.
Due to that multiple tests for differential expressions were performed, the false discovery rate (FDR) was determined.
The sub-cultured cells were harvested at 48 h after plating, and analyses for gene expressions were performed.
The correlation of NP T2 and the Thompson grading and linear regression of NP T2 versus gene expressions were performed.
Statistical analyses of API5 and pERK1/2 expressions were performed using the Mann–Whitney test or the Kruskal-Wallis test.
Data normalization, background correction and all subsequent statistical tests for significant differential expressions were performed by using a Partek statistical software package (Partek., Inc, St . Louis MO).
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Seven facial expressions are performed at four intensity levels by each subject.
Validation of the two explicit expressions is performed by both comparison to experimental data and comparison with validated numerical simulations.
Correlation between different gene expressions was performed with Pearson correlation coefficient method.
b The relative levels of phosphorylated p38, ERK1/2, and JNK expression were performed by densitometric analysis.
b, c The relative levels of COX-2 and iNOS expression were performed by densitometric analysis.
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