Sentence examples for expressions were designed from inspiring English sources
9 11 Regular expressions were designed to identify novel miRNas with, and without, species identifiers (e.g. hsa-miR-1 and mir-1).
An optimization routine, called a feedback scheduler, that uses these expressions is designed.
miRNAs for the specific knockdown of rat TrkB-T1 expression were designed using the RNAi-designer website from Invitrogen (Carlsbad, CA, USA).
Primers used for GAPDH mRNA expression were designed as follows: [GAPDH-F: 5′-CGACCACTTTGTCAAGCTCA-3′ GAPDH-R: 5′-GAGGGTCTCTCTCTTCCTCT-3′], while those for CYP24A1 [CYP24A1-F: 5′-TGAACGTTGGCTTCAGGAGAA-3′,CYP24A1-R: 5′-AGGGTGCCTGAGTGTAGCATCT-3′] were adopted from Yosuke61 et al. 2009.
Within this study two systems promoting inducible expression were designed on the basis of the strong j5 promoter and the Escherichia coli lacI or the Pseudomonas putida cumate regulatory elements.
Primers used for analysis of gene expression were designed by use of NCBI Primer-BLAST (https://www.ncbi.nlm.nih.gov/tools/primer-blast/) and are in Table 1.
Antisense morpholino-oligonucleotides to knock down endogenous MEK1 (MEK1-AS) expression were designed and produced by Gene Tools LLC (Philomath, Oregon, USA).
The primer pairs for gene expression were designed to target exon-exon junctions if the target gene contains multiple exons.
Specifically, the probes used to investigate AmNrxI-A and AmNrxI-B expression were designed to the common 5' region of the gene.
In all subsequent experiments, primers to quantitate OXR1 mRNA expression levels and dsRNA constructs to silence gene expression were designed from the TLDc domain, which is common to all OXR1 isoforms.
Oligonucleotide probes for microarray analysis of gene expression were designed with CombiMatrix probe design software (Probe Weaver) based on: (i) uniqueness within a defined gene and sequence set; (ii) Tm and length within a specified range; 70 75°C, and 35 40-mer 35 40-mervely; and (iii) absence of stable secondarespectivelys and repeat sequences.
