In fact, previous studies targeting calcineurin expression were conducted to try to promote slow MyHC positive fibers, although only in the less severe (with respect to Sgca null mice) dystrophic mdx mouse model32, 36.
Rubisco expression were conducted by 0.2 mmol/L IPTG for 6 h at 22°C.
To confirm and extend the results shown in Figure 1a, quantitative real time RT-PCR analysis of PHD3 mRNA expression were conducted and the results are shown in Figure 1b.
Statistical tests of differential expression were conducted using the moderated t-test in Bioconductor.
Normalization and statistical analysis of miRNA expression were conducted using SABiosciences Online PCR Array Data Analysis Web Portal.
Based on these data, further analyses of changes in gene expression were conducted on cells collected 48 h after transfection.
The expression data and predicted target site information were merged and correlations between miRNA and mRNA expression were conducted using the open source statistical program R [ 70].
Further analyses of transcriptomic changes associated to miR-155 and miR-802 expression were conducted to identify those predicted mRNAs more likely to be directly regulated.
Because the different strains have transcriptional profile clustering based on the culture model in which it was propagated, thorough studies of gene expression were conducted.
