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HMEC oncogenic data [ 22] was downloaded from Gene Expression Omnibus (GEO) [ 23] and pathway expressions were calculated as mean of differentially expressed genes with induced genes weighted positively and inhibited genes negatively.
Relative expressions were calculated by the formula 2−∆∆Ct.
Kinetic expressions were calculated from the reaction rate data obtained from the tube reactor by least squares fitting to general power law model, as well as to a mechanism-based model put forth by Peppley et al. [Appl. Catal. A 179 (1999) 21; Appl. Catal. A 179 (1999) 31] assuming isothermal plug flow behavior.
Relative expressions were calculated using the method published by Pfaffl, M [25].
Thermal cycling was carried out in an iQ 5 (Bio-Rad) and relative expressions were calculated following previously described methods [51].
Relative expressions were calculated by method.
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Corresponding to user's nature, optimal power expressions are calculated.
Fold changes in gene expression were calculated by ΔΔCT method.
Changes in expression were calculated via the ΔΔCt method.
Fold change in gene expression were calculated as 2 -ΔΔCt.
Fold changes in expression were calculated using the ΔΔCt method.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com