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Typically four 175 cm flasks were used per SWS1 expression procedure, with one flask of similarly expressed bovine rhodopsin as a control.
It should also be emphasized that this expression procedure in wild type background did not lead to division phenotypes.
The yeast expression procedure has been described previously (33).
6×His-Gαo1 was produced by the autoinductory expression procedure [25].
The SAFE (Significance Analysis of Function and Expression) procedure was used to identify molecular pathway perturbations.
The yeast cells harboring empty vector were subjected to the same expression procedure and reaction conditions were used as control.
Supplementary figures, supplementary tables, and supplementary methods, containing a detailed description of the expression procedure, crystallographic work, enzymatic tests, and thermal denaturation assays.
To compare between two general tissue types that are of interest for woody biomass production [ 27] such as stem and branch, along with the comparison between young (12-years-old) and mature (60-years-old) trees, we properly performed the differential expression procedure with DESeq program (Additional file 4, Fig. 2).
Recently, Kwon et al. (Kwon et al. 2010) established the cell-free expression of computationally predicted putative ω-TAs, which circumvents cloning and expression procedures.
Because VEGF is the major player in both angiogenesis and vascular permeability and the most explored factor in angio-inhibitory therapies, many expression procedures have been developed to produce functional VEGF165 in convenient yield.
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