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I had never listened to the band growing up, but I remembered seeing the band's T-shirts on girls who looked like they could yank their pensive Winona Ryder expressions off of their translucent faces with one tug of their lip ring.
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Nonetheless, both expressions, off-line and on the fly, are also used with other connotations.
It is important to keep gene expression OFF while isolating a cell line stably expressing a gene of interest, because the expression of certain proteins may interfere with cell growth and thus with the process of isolating stable clones.
This element may play an important role in keeping Olig2 expression off in undifferentiated ES cells.
Interestingly, all switches in fucosyltransferase activity observed in the isolates resulted in an on-switch rather than turning the Lewis antigen expression off.
We attempted to shut HOXB4 expression off in recipient mice from 8 to 14 weeks after transplantation by letting them drink water containing 100 µg/ml of Dox.
Administration of Tc or dox switches gene expression off (Tet-Off system).
However, we have not found any evidence for differential expression of major mediators of the Notch or Wnt pathways in the DoxHPC lines after turning Lhx2 expression off.
As expected, in the activator configuration, transgene expression is ON in the absence of cumate, which for certain applications would require culturing cells in the presence of cumate to keep transgene expression off until appropriate.
Etv5 and Csrp2 (cysteine and glycine-rich protein 2), which also showed significant decrease in expression after turning Lhx2 expression off, have been implicated in mesenchyme-epithelia interactions during embryogenesis [ 43, 44], similar to Lhx2.
In the absence of signal, the TCF family member is bound at the promoters of Wnt-regulated genes, where it interacts with transcriptional repressors to keep target gene expression off or low.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com