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The relative expressions of target genes were calculated using the 2ΔC t) method.
Expressions of target gene from these plasmids in HeLa cells were verified by Western-blot.
Gene Ontology analysis results for target mRNAs suggested that sex- and phase-specific miRNAs in the locust brains may be involved with the polyphenisms by regulating the expressions of target mRNAs in various physiological functions.
Relative expressions of target genes were determined by the 2−ΔΔCt method [25].
The mRNA expressions of target genes were measured by qPCR with the Roche LightCycler® 480 System (Roche Applied Science, Mannheim, Germany).
For primer sequences please see table 1. Relative expressions of target genes were normalized to eukaryotic translation elongation factor 2 (EEF2), analyzed by delta-delta-Ct method and given as percentage compared to control experiments.
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This newly recognized feature shows us an additional way that regulatory proteins can direct expression of target genes — in this case by directing expression of genes encoding these microRNAs.
The expression of target genes was normalized to the internal reference ubiquitin.
The expression of target gene can therefore be repressed by anhydrotetracycline.
Wnt signals regulate the expression of target genes by activating a conserved signal transduction pathway.
To elicit cytolysis of tumor cells, effector T cells rely on tumor expression of target antigens.
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