Further investigation should be carried out to establish transformed insect cell lines with stable expression of corresponding interfering RNAs.
The result of dual-luciferase reporter assay and qRT-PCR showed that alncRNAs could inhibit transcriptional activity of TFs and expression of corresponding microRNAs.
NATs can affect the expression of corresponding sense genes in cis or in trans at multiple levels (Pelechano and Steinmetz, 2013).
The expression of corresponding miRNA target genes was measured using TransScript II Green One-Step qRT-PCR SuperMix (Transgen) with beta-actin and GAPDH as internal controls.
Many lncRNAs are involved in gene expression regulation with neighboring genes as targets, so the expression of corresponding neighboring genes (ZEB1 vs. ZEB1-AS1; PTENP1-AS1 vs. PTENP1, DICER1 vs. DICER1-AS1, MORC2 vs. TUG1, HOXC11 vs. HOTAIR) was examined.
While ExMV-derived mRNAs may be translated in target cells into appropriate proteins, miRNAs regulate expression of corresponding mRNA species, and both RNA-depended ExMV-mediated mechanisms lead to functional changes in the target cells.
However, regardless of higher silique carbohydrate content in HOCL, it is not certain that expression of corresponding carbohydrate related genes are really higher than that in LOCL during rapeseed seed development.
Next, we measured the expression of corresponding chemokines in prostates during regrowth, to determine a mechanism for MSC homing.
Allele-specific gene silencing by RNA interference (RNAi) is therapeutically useful for specifically inhibiting the expression of disease-associated alleles without suppressing the expression of corresponding wild-type alleles.
ASP-RNAi would thus be therapeutically very useful, as it can specifically suppress the expression of alleles causing disease without inhibiting the expression of corresponding wild-type alleles [10] [17].
