Transient transfections were performed with FUGENE 6 transfection Reagent (Roche), using 120 ng of luciferase reporter plasmid co-transfected with 40, 80 and 100 ng expression vector, in accordance to manufacturer's instructions.
For cbhB this expression pattern is in accordance to what has been observed previously (Delmas et al., 2012).
To achieve a high expression of antigen after genetic vaccination, HPV 16 E5 gene was codon-optimized for the expression in mammalian cell in accordance to previous studies, showing 6 to 9-fold enhanced expression by codon optimization.
In the present study, cells sampled from SCS-BTA18-Q animals exhibited corresponding changes in gene expression after pathogen challenge in accordance to other studies investigating molecular mechanisms of immune response in mammary gland epithelial cells [ 6, 8, 47].
Quantitative real-time PCR showed an upregulation of VE-cadherin expression exclusively in cEND cells, in accordance to previous observations made for the occludin gene.
The expression of all five miRNAs was in accordance to microarray results in FFPE, when RNU6B was used as RG.
Statistical analysis revealed that miR-1, miR-133a/b and miR-98 expression based on qPCR results is in accordance to microarray results, that are down-regulated in infracted compared to corresponding remote myocardium, but statistical significance is dependent of RG used (Table 5).
In this manner, expression patterns of virulence genes may differ in accordance to their host.
Do we have a semantic obligation to use our expressions in accordance with their meaning?
Indeed, we arrive at mathematical equations by "the method of substitution": "starting from a number of equations, we advance to new equations by substituting different expressions in accordance with the equations" (6.24).
