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Equal amount of total protein was loaded on a 10% Tris-HCl Rgel for expressions analysis by Western immunoblotting.
The BioMark Dynamic Array microfluidic qPCR system (Fluidigm Corporation, San Francisco, CA) was used for gene expressions analysis of oocytes and cumulus cells.
Fifty genes with the highest and lowest combined score as well as their matching fold changes identified in the gene expressions analysis are shown in Supplementary Table 4.
In order to validate the results obtained by the differential expressions analysis in our dataset, we perform differential analysis on two independent datasets.
Expressions analysis with real-time PCR showed that ASS1 was barely detectable in either the healthy tissues or the calluses of A. sinensis, whereas in the wounded tissues or MJ-treated calluses, ASS1 expression was increased almost 1000-fold (see Figure 6).
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Gene expression analysis showed 19 differentially expressed genes.
Expression Analysis Highlights AXL as a Candidate Zika Virus Entry Receptor in Neural Stem Cells.
NtNAR gene expression analysis.
Gene expression analysis.
Figure 1 Gene expression analysis.
Fig. 4 Expression analysis of OsHox32.
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