We employed the Pichia pastoris secretory expression system to express ARG I and the expression yield of it was as high as ~1510 mg/L.
We have achieved a high level of expression yield of rhTF in rice (Oryza sativa L .. Expression yield is 40% of total soluble protein or 1% seed dry weight (10 g/kg) [ 25].
Yu et al. (2016) optimized pressure, electric field strength, and pulse number on the juice expression yield, total polyphenols, and total proteins content in the expressed juices of rapeseed stem biomass.
Thus, in this study, we successfully expressed the xylanase gene xynZG in K. lactis despite of expression yield in K. lactis was lower than that in P. pastoris.
Nevertheless, the intron sequence of atTOC120 and atTOC90 indeed increased the expression yield obviously.
This is probably due to the low expression yield of atTOC120 in Arabidopsis.
Replacing the GGD in (A.1) and manipulating the obtained expression yield (A2). where.
There are great demands on the stability, expression yield and resistance to aggregation of antibody fragments.
We find that the initial expression yield can be enhanced twofold to threefold in this manner.
However, the intrinsic toxicity of AMPs induces their proteolysis in host cells and inhibits cell growth, thus limiting expression yield.
The biodiesel yield was calculated by the following expression: Yield = (weight of biodiesel × % FAME /(weight of oil) × 100%.
