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Changes in gene expression were verified by semiquantitative analysis.
The cells were cultured for an additional 48 h and the effects of these treatments on hsp90β1 expression were verified by western blot analysis as described [17].
Changes in expression were verified by real-time qPCR with a correlation of >0.95 to the microarray data.
The functional differences in MEC subpopulations identifiable by MMTVrtTA/H2BGFP expression were verified by gene expression analysis.
We could not identify any problem in the alignment of reads in those regions, but none of the SNPs exhibiting B only expression were verified by Sanger sequencing.
After 24 h, cells were extracted in 0.5% NP40 and homogenized, and the levels of UBE3A expression were verified by western blotting.
Similar(53)
Back-complementation cell lines expressing D366N (LEDGF/p75D366N) or WT LEDGF/p75 (LEDGF/p75BC) were generated and their expression was verified by western blot (Fig. 3B).
Expression of fusion protein expression was verified by western blot (Supplementary Fig. 8d, e).
The expression is verified by experimental results and considers the steel fiber enhancement and weakening effect.
HEK293 cells were transfected with GFP-SERCA2a and RFP-PLB constructs, and expression was verified by SDS-PAGE and immunoblot (Fig. 2a).
The reduction of C1s mRNA by shRNA expression was verified by qPCR, displaying 47.4±8% reduction in the levels of C1s as compared to control shRNA (n=5, Student's t-test, P=0.0067).
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