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Selected proteins as NPM1, RKIP, HSP90B1 and NCL were also differentially expressed among the analyzed subgroups and these levels of expression were validated by western blotting of pooled samples (Fig.  2).

Results of gene expression were validated by RT-PCR.

Changes in gene expression were validated by real time PCR using TaqMan assays, values in brackets are relative to those for the eGFP controls, for comparison.

Selected genes that responded to doxorubicin or lovastatin treatment with relevant changes in mRNA expression were validated by performing additional real-time RT-PCR analyses (triplicate determinations).

Moreover, changes in the pattern of gene expression were validated by qRT-PCR, including cytokines, cytokine receptors, and transcription factors, apoptosis-associated genes, and interferon related genes.

The effects of CpG island hypermethylation of the BRCA1 promoter on expression were validated by IHC analysis for BRCA1 gene products on tissue microarrays (TMAs) demonstrating a significant association (Table 1 ).

The knockdown efficiency of siRNAs on p65 and p50 gene expression was validated by qRT-PCR (Supplementary Figure S6g).

In Part II, the proposed mechanism and flame propagation speed expression are validated by numerical simulation.

iGFP-WT (clone #12.5) and iGFP-NLSm (clone #6.B7) individual subclones were expanded and transgene expression was validated by IF and immunoblot.

The accuracy of the analytical expression is validated by comparing it with the step-by-step simulation results.

This expression is validated by a series of numerical experiments using 1D and 2D high resolution landscape evolution models.