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Comparisons between tumour tissue PIF-CP gene expression and benign tissue expression were tested by linear regression analysis following natural logarithmic transformation of the data.
Selected genes for which replicate microarray analyses consistently showed differing degrees of VPA-dependent change in expression, were tested by RT-qPCR.
Differences in allelic expression were tested by t-test and analysis of variance (ANOVA) by use of GraphPad Prism v5.01 (GraphPad Software, La Jolla, CA, USA).
To identify a clinically meaningful cut-off for Aurora A mRNA expression, various levels of Aurora A mRNA expression were tested by the Kaplan-Meier method and verified by the log-rank test.
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To detail the melanocyte populations expressing the transgene, PAX3 expression was tested by immunohistochemical staining in hair follicles and epidermis of wild-type and PAX3-tg mice.
Significance of differential expression was tested by an empirical Bayes moderated t-test and adjusted for multiple-testing using Benjamini and Hochberg's method (Benjamini et al, 2001).
Difference in gene expression was tested by Wilcoxon Rank test (p < 0.05).
Cas9 expression was tested by immunoblot using 4 12% Bis-Tris SDS polyacrylamide gels (used for all immunoblot applications in this work).
To determine if differential expression of OsGLP family members correlates with disease phenotypes, gene expression was tested by RT-PCR on a time course (0, 12, 24, and 48 hpi) after M. oryzae infection (Fig. 3).
Consequently, although the specific activity of both enzymes was improved successfully by indirect induction of chaperones or decelerated proliferation and expression rates, a further improvement of soluble expression was tested by coexpression of five different chaperone sets (see Additional file 1).
Gene expression was tested by real time RT-PCR.
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