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Surface and total expression were measured by adding α-flag FITC with and without Triton, respectively.
Bcl-2, Bax, Bcl-xL, and β-actin mRNA expression were measured by quantitative real-time RT-PCR in an MX3005p PCR system (Stratagene, Europe).
Type-collagen-I (Col1), type-collagen-III (Col3) and α-smooth muscle actin (α-SMA) expression were measured by western blot and real-time quantitative polymerase chain reaction.
Therefore, TNFR1, Fas, and FasL expression were measured by using flow cytometry analysis.
ZNF93, ZNF43, and THADA RNA expression were measured by TaqMan RNA assay kit.
Aromatase and SF-1 expression were measured by RT-qPCR in KGN and COV434 cells, following separate and combined treatment with forskolin (FSK) and phorbol myristate acetate (PMA) (Fig. 1B and 1C).
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PD-L1 expression was measured by flow cytometry.
LOX-1 mRNA expression was measured by RT-PCR.
In peripheral blood monocytes DDAH2 mRNA expression was measured by quantitative PCR (qPCR).
Cerebral DDAH-1 mRNA expression was measured by means of RT-PCR.
B Islets and acinus were isolated from the mouse pancreas, and PI4KIIα expression was measured by Western blot.
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