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The parameter values in the rate expression were estimated by non-linear regression analysis.
Relative levels of expression were estimated by densitometry.
Differences in gene expression were estimated by t-test.
The reduction in Beclin1 and KOR mRNA expression were estimated by real-time PCR analysis.
The levels of both Bcl-2 and Bax mRNA expression were estimated by using quantitative PCR analysis.
Differences in gene expression were estimated by EST frequency for genes with at least four ESTs present in the dataset using the web tool "Identifying Differentially Expressed Genes 6" (IDEG6; (http://telethon.bio.unipd.it/bioinfo/IDEG6_form/) [ 53] with the recommended chi-squared test for multiple library comparisons with a p-value cut-off of < 0.0001.
Similar(53)
Unigene expression was estimated by the FPKM method, and differentially expressed genes were identified by referenceto Audic [ 21].
Metalloproteinase (MMP) expression was estimated by immunohistochemical analysis.
The relational expression was estimated by statistical method on explanatory variable and objective variable about hydrolysis efficient by each enzyme.
Relative expression was estimated by counting the total number of ESTs for each FBX locus.
γ-H2AX expression was estimated by anti-phospho-histone H2AX (Ser1∶100(1∶100) antibody and donkey anti-mouse IgG-FITC.
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