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Initially, the three patient-specific biological replicates were each tested using Student's one-sample t-test and their changes in gene expression were defined by fold change relative to the gene expression level in self-hybridization assay; this analysis was performed with a two-fold change filter applied and no multiple testing correction or statistical significance filter of p < 0.05 adopted.
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In maize, RLD1 encodes an HD-ZipIII protein whose adaxial expression is defined by miRNA166-directed transcript cleavage on the abaxial side, which is an important determinant of adaxial cell fate during maize leaf development (Juarez et al. 2004).
FUNC, the Sum Rule and the Product Rule, as restricted to one measurement context, trivially repeat these definitions, and there obviously is no point in testing, e.g., whether v(A·B) really equals v(A) v(B), if the former expression is defined by the latter.
The legal framework regulating French freedom of expression is defined by several amendments, and while it remains an almost unfettered right, there are laws in place to determine and prevent abuses.
For correlation coefficient analysis and the generation of Venn diagrams, detected gene expression was defined by a Detection P-Value <0.01.
For all microarray data except the dam mutant, differential expression was defined by a q-value of 0.05 following FDR multiple testing.
Unless stated otherwise, differential gene expression was defined by a minimal three-fold difference (rounded to one decimal place) in average normalized tag counts (TPM) between any two datasets being compared.
A high CA9 and VEGF expression was defined by an expression score ≥ 80.
Consequently, over expression was defined by R1 and D1 and under expression by R2 and D2.
Positive expression was defined by staining of more than 1% of the tumor area.
MicroRNA expression was defined by ISH staining scores, as described previously (Acs et al, 2003).
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