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Furthermore, protein data of inversely expressed Bax and Bcl-2 protein expression were confirmed by additional analysis of Bax and Bcl-2 mRNA expression using real-time PCR (data not shown).
The expressed products associated with probes indicating differential expression were confirmed by qRT-PCR.
Changes in actin, tubulin and myosin expression were confirmed by flow cytometry of parasite cells and immunofluorescence.
Subsequent studies were made only with genes whose differences in levels of expression were confirmed by these assays (Fig. 1).
Our findings of a diminished TRPC1-, TRPC4- and TRPC6-channel expression were confirmed by immunohistological stainings of punch biopsies from psoriasis plaques.
HLA-A, -B and -C surface expression and the lack of expression were confirmed by using the traditional CDC assay, using allo-antisera in Terasaki trays, as previously described with some minor modifications [26].
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Seed-specific expression was confirmed by expressing the gus gene in Arabidopsis.
Protein expression was confirmed by western blotting.
In addition, mCherry expression was confirmed by western blotting (Supplementary information, Figure S2D).
Transgene expression was confirmed by RT-PCR of embryonic skin RNA using the genotyping primers.
This reduction in BMPR-IA expression was confirmed by Western immunoblot and immunohistochemistry in the flox/flox group.
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