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Mean values of DKK1 expression were compared by clinico-pathological clusters with the two-tailed t-test for independent samples.
Animal characteristics and myocardial protein expression were compared by use of 1-way ANOVA, followed by a Fisher LSD post-hoc analysis.
The clinicopathologic factors in various groups of patients with negative or positive TCF4 expression were compared by means of χ test or Student's t-test.
Body weight, left ventricular weight, the size of the area at risk and infarct size, and protein expression were compared by using analysis of variance (ANOVA) with Sidak corrections for multiple comparisons.
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HA-TPβ expression was compared by one-way ANOVA with the Bonferroni post test.
Conservation of gene expression was compared by checking the 3,892 1∶1∶1 orthologous genes in mouse, chicken and frog (Table S5).
RNA was prepared from the ventricles of postnatal day 17 miR-499 transgenic mice or littermate controls, and gene expression was compared by microarray analysis (Fig. 5A).
Expression was compared by colocalization with mouse monoclonal antibody 22C10, which marks neurons and the abdominal founder myoblasts very clearly [11].
In spheroid-derived cells (SDC) and the parental monolayer-derived cell line ALDH1, CD44, CD24, E-Cadherin, α-SMA, and Vimentin expression was compared by flow-cytometry and immunofluorescence together with proliferation and cell cycle analysis.
Then, telomerase gene expression was compared by real-time PCR in two groups.
Gene expression was compared by DNA microarray hybridization (GEO code GSE54712).
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