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After four days, proliferation and CD25 expression were assessed by flow cytometry.
Gene and protein expression were assessed by quantitative real-time RT-PCR and immunoblot.
T-cell proliferation (Ki-67) and function (IFN-γ, TNF-α, IL-2, CCL4, and CD107a expression) were assessed by multi-parameter flow cytometry.
Securin and cathepsin D expression were assessed by immunohistochemistry in an independent set of 20 prostate, 47 breast, 20 lung, and 11 ovarian cancer samples.
H19 and α-fetoprotein expression were assessed by using both radioactive and non-radioactive probes for the expression of the H19 gene and immunohistochemistry, for the α-fetoprotein expression according to Ariel et al. [12].
The significance of the different treatments on C-term-pSMAD1 localisation and on Olig2 expression were assessed by one-way analysis of variance (ANOVA) followed by Tukey HSD test or t-Test with Bonferoni correction, as indicated in the text.
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ATDC expression was assessed by Western blotting.
Gene expression was assessed by Illumina microarrays.
EGFP expression was assessed by flow cytometry.
Renal preproET-1 expression was assessed by Northern blot analysis.
The BCL2L11 expression was assessed by Western blotting analysis and samples were normalized to GAPDH.
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