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Clustering and annotation of the gene expression were analyzed by using Cluster and TreeView [ 20], Onto-Express [ 21], and GenMAPP [ 22].
Gene and protein expression were analyzed by qRT-PCR and Western blot respectively.
Changes in pulmonary miRNA expression were analyzed by comparing OVA/OVA exposed mice with control mice (PBS/OVA) via locked nucleic acid (LNATM) microarray (Exiqon, Vedbaek, Germany).
Type 1 and type 2 inflammatory cytokines (TNFα, CCL18) secretion and histological biomarkers (CD206, stabilin-1) expression were analyzed by ELISA and confocal microscopy respectively.
Conditioned media containing all secreted factors was harvested at day 1, 3 and 7. Chemokine secretion and expression were analyzed by Chemi-array, ELISA (Macrophage migration inhibitory factor (MIF), plasminogen activator inhibitor type 1 (PAI-1)) and RQ-PCR.
Data of relative expression were analyzed by Student's t-test.
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(A) From liver tissue, UGT1A1 and Cyp2b10 gene expression was analyzed by real time PCR and expressed as Fold induction.
Icer expression was analyzed by qPCR.
Protein expression was analyzed by IB.
The fold change of gene expression was analyzed by RT-qPCR as described previously51.
Sox2 expression was analyzed by immunohistochemistry and compared to clinicopathologic features, time-to-progression (TTP) and overall survival (OS).
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