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Relative changes in gene expression were analysed via the 2ΔΔC(T) method.
Protein phosphorylation and expression were analysed by immunoblotting (images with black bands) (a) and quantified (b).
Protein phosphorylation and expression were analysed by immunoblotting (c,g) and quantified by densitometry (d,h).
The changes in the pattern of gene expression were analysed by means of DNA/RNA hybrid antibodies in the polytene chromosomes from salivary gland cells.
The relative changes in gene expression were analysed by the 2−ΔΔCT method [57].
Probesets with a 5-fold or more change in expression were analysed with Ingenuity Pathway Analysis (IPA), a list of 268 eligible entities for IPA analysis.
Ctnna1 (α-catenin) and Ctnnb1 (β-catenin) gene expression were analysed using the ABI PRISM 7900HT instrument and samples normalised to expression of endogenous control.
In order to address if inhibition of transcription would affect the expression of spindle checkpoint transcripts, bubR1, bub3, mad1, mad2 and cdc20 gene expression were analysed by real-time quantitative PCR.
The Cre recombinase was activated in R26cre-ert/+; β-cateninflox/flox embryos at E8.5 or E9.5 and the effects of gene expression were analysed 1 day later at E9.5 or E10.5.
Levels of protein expression were analysed by western-blot.
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Levels of CD4 and CD8-expression were analysed flow cytometrically ex vivo and after stimulation with PMA at day 1, day 7 and day 14 in culture (proliferation index).
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