Back-complementation cell lines expressing D366N (LEDGF/p75D366N) or WT LEDGF/p75 (LEDGF/p75BC) were generated and their expression was verified by western blot (Fig. 3B).
Expression of fusion protein expression was verified by western blot (Supplementary Fig. 8d, e).
HEK293 cells were transfected with GFP-SERCA2a and RFP-PLB constructs, and expression was verified by SDS-PAGE and immunoblot (Fig. 2a).
The reduction of C1s mRNA by shRNA expression was verified by qPCR, displaying 47.4±8% reduction in the levels of C1s as compared to control shRNA (n=5, Student's t-test, P=0.0067).
Expression was verified by SDS-PAGE and western blot (Fig. 2b), showing that after IPTG induction for 20 h and only in the 2YT medium, a protein was over-expressed.
Expression was verified by RT-PCR.
Protein expression was verified by SDS-PAGE analysis.
Inhibition of FBXW8 expression was verified by a RT-PCR.
HCNCp mRNA expression was verified by Northern analysis.
Protein expression was verified by an immunoblot (Fig. S7).
LRP1 expression was verified by both immunocytochemical staining (Figure 4c, d) and Western blotting (Figure 4e).
