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Gene expression was validated by real time PCR.
Stable miR-200a expression was validated by RT-PCR (see below).
Differential gene expression was validated by quantitative RT-PCR, Western blot and immunofluorescence.
Differential expression was validated by qPCR in randomly selected genes (listed on Table S1).
Differential gene expression was validated by quantitative real time RT-PCR.
Differential expression was validated by real-time RT-PCR using the Roche LightCycler 480.
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Selected proteins as NPM1, RKIP, HSP90B1 and NCL were also differentially expressed among the analyzed subgroups and these levels of expression were validated by western blotting of pooled samples (Fig. 2).
In Part II, the proposed mechanism and flame propagation speed expression are validated by numerical simulation.
The accuracy of the analytical expression is validated by comparing it with the step-by-step simulation results.
This expression is validated by a series of numerical experiments using 1D and 2D high resolution landscape evolution models.
The new generalised expression is validated by showing it is consistent with specific cases from past literature.
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