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This candidate was mostly expressed in the cancer cell line and its expression was validated in clinical samples, indicating a possible role in cancer.
The expression was validated through simulations showing a perfect agreement between exact and simulated curves.
The new expression was validated against experimental test results of RC beams strengthened using prestressed Near Surface Mounted (NSM) carbon FRP un-fatigued and fatigued beams.
Gene expression was validated by real time PCR.
Production of lentivirus was carried out and PrP expression was validated in Prnp−/− cells.
Differential gene expression was validated by quantitative RT-PCR, Western blot and immunofluorescence.
Differential gene expression was validated by quantitative real time RT-PCR.
Differential expression was validated by real-time RT-PCR using the Roche LightCycler 480.
Cell surface cytokine expression was validated by further intracellular detection using the Cytofix/Cytoperm reagent according to the manufacturer's instructions.
Similarly, an increase in dmp-1 expression was validated in the PyV MT/jnk2−/− compared to PyV MT/jnk2+/+ tumors (data not shown).
The GFP expression was validated by FACS, and SphK-1 overexpression was confirmed by western blot, immunofluorescence staining and competitive S1P titre (Fig. 2A).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com