To detail the melanocyte populations expressing the transgene, PAX3 expression was tested by immunohistochemical staining in hair follicles and epidermis of wild-type and PAX3-tg mice.
Significance of differential expression was tested by an empirical Bayes moderated t-test and adjusted for multiple-testing using Benjamini and Hochberg's method (Benjamini et al, 2001).
Difference in gene expression was tested by Wilcoxon Rank test (p < 0.05).
Cas9 expression was tested by immunoblot using 4 12% Bis-Tris SDS polyacrylamide gels (used for all immunoblot applications in this work).
This heterologous expression was tested in several E. coli mutant backgrounds with increased availability of C4 intermediates.
Cluster/cell type-specific differentially RNA expression was tested using a nonparametric binomial test17, which compares the frequency of gene expression in the cluster with its frequency in all the other cells.
To determine if differential expression of OsGLP family members correlates with disease phenotypes, gene expression was tested by RT-PCR on a time course (0, 12, 24, and 48 hpi) after M. oryzae infection (Fig. 3).
Gene expression was tested by real time RT-PCR.
Light induced endogenous gene expression was tested by real time qPCR.
The possibility that the inhibitory antibodies might differentially modulate cell surface 5T4 expression was tested.
The potential effect of antipsychotic drugs on Sprouty2 expression was tested in adult rats.
