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Hsp90 α and β expression was quantitated by SDS PAGE followed by quantitative western blotting on serially diluted cell extracts.
Confluent primary fetal and postnatal fibroblast cultures were stimulated with TGF-beta1 for 24 hours, and lysyl oxidase expression was quantitated by performing real-time polymerase chain reaction.
Its expression was quantitated by Northern analysis in separated uninfected amnion, chorion, decidua, and placenta of patients at term before labor and in full-thickness membranes before and after spontaneous labor at preterm and at term.
To explore whether alleviating the chemically induced leukemia by the plant mixture extract involves regulation of sphingosine-1-phosphate receptor-1 (S1PR1), lymphocytes were isolated from the "untreated," "carrier," "DMBA," "plants after DMBA," and "plant mix" rat groups and S1PR1 mRNA expression was quantitated by real-time PCR.
Activation marker expression was quantitated by flow cytometric analysis.
PCR was then performed on the cDNA using standard conditions with primers for rtTA2s-M2 (5' CTGTGTCandAAGGCTCAGCAGGCAGCATATCAAGGCAGCATATCAAG) EGFP expression was quantitated by fluorimetry as described by Yata et al. [43].
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Binding of the antibody was detected by an ImmunoStar Zeta (Wako Pure Chemical), and levels of protein expression were quantitated by a luminoimage LAS-4000 analyzer (Fuji Film, Tokyo, Japan).
The β-actin (Actb) mRNA level was used as an internal reference, and levels of mRNA expression were quantitated by optical densitometry after electrophoresis on an agarose gel.
Relaxin gene expression was quantitated from the arrays and in additional tissues by Northern analysis.
Protein expression level was quantitated by densitometric analysis as previously reported [ 28].
The relative intensity of the procaspase expression level was quantitated by densitometry.
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