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To determine which of our primary candidate genes for Fatq2b were differentially expressed, gene expression was quantified in Atp5e, Ctsz, Gnas, Rab22a and Stx16, and two housekeeping Gus and SDHA.
Luciferase expression was quantified in live cells for 8 weeks, revealing persistence of gene expression.
GILZ protein and gene expression was quantified in blood neutrophils, along with markers of inflammation (CRP, extracellular DNA) or its resolution (Annexin A1).
Gene expression was quantified in triplicates as previously described [24].
Induced gene expression was quantified in cell culture (fluorescence microscopy) and in vivo (BLI, PET).
In a first series of experiments, miRNA expression was quantified in right and left colon from healthy control subjects.
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RICTOR, MAPKAP1 and mTOR expression were quantified in triplicate.
Both XIST and KDM5C expression were quantified in the cingulate cortex.
Expression is quantified in FPKM (fragments per kilobase of transcript per million).
AhR, CYP1A1 and cdc42 mRNA expression were quantified in AhR by real-time PCR.
The expression is quantified in terms of reads per kilobase of exon per million (RPKM fragments mapped).
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