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DAXX expression was quantified by densitometry analysis.
Endogenous versus exogenous gene expression was quantified as previously reported65.
Protein expression was quantified using Image J (NIH, USA).
Surface protein expression was quantified using flow cytometry.
Gene expression was quantified and differential expression was calculated with Cufflinks.
Luciferase expression was quantified in live cells for 8 weeks, revealing persistence of gene expression.
TNFα and TNFR2 protein expression was quantified as previously reported using Elisa R&D kits26.
Spp1 mRNA expression was quantified by RT-qPCR as described in Materials and Methods.
IBSP gene expression was quantified by qPCR after the entire 3 weeks of culture.
LPL gene expression was quantified by qPCR after the entire 3 weeks of culture.
ALPL gene expression was quantified by qPCR after the entire 3 weeks of culture.
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