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Bioreactor glucagon expression was performed.
Gene expression was performed using RT-PCR.
Immunohistochemistry for neurofilament expression was performed.
The protein expression was performed as described previously [7].
Transient expression was performed according to Wang et al. (2010).
The styB expression was performed under similar conditions as described for styA's.
Gene expression was performed using a reverse transcriptase polymerase chain reaction (RT-PCR).
Knockdown of renin/prorenin receptor expression was performed by small interfering RNA (siRNA) transfection.
Immunohistochemical staining for protein expression was performed on the processed monolayer slides.
Quantification of gene expression was performed using a real-time PCR system (7900HT Fast Applied Biosystemss) in triplicate.
Then the cultures were induced with 1 mM IPTG and the expression was performed at 28 °C for 18 h.
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