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TIEG1−/− osteoclast precursors were infected at day 2 with either adenovirus expressing TIEG1 or vector alone and TIEG1 expression was monitored by QPCR at day 3 and 4 to determine relative TIEG1 expression levels.

Twenty-four hours after injection, GFP expression was monitored by fluorescence microscopy, and the ratio of cells expressing GFP to Texas Red positive cells was calculated.

The transformed plants were confirmed by PCR (data not shown) and the transgene expression was monitored by real time RT-PCR for a transgenic line ectopically expressing the wild type protein (NIK*), two independently transformed KO lines expressing the G473V/T474A mutant protein (mNIK-A and mNIK-B), and transformed lines expressing T469A or T474A mutant proteins (Figure 8A).

Bim expression was monitored by Western blotting with anti-Bim pAb.

Gene expression was monitored by RT-qPCR using the SYBR Premix Ex TaqII (TaKaRa).

CFTR expression was monitored by reverse-transcription polymerase chain reaction, immunostaining, and Western blotting.

Gene expression was monitored by RT-qPCR, carried out using SYBR Green PCR master mix (Tiangen-bio, Beijing, China).

Transgene expression was monitored by the chlorophenol-red assay for pmi and SDS-PAGE of seed proteins for 1Dy10.

The L. lactis MG1363 (pExu:egfp) strains were administered by gavage to Balb/C mice and the eGFP expression was monitored by fluorescence microscopy.

Reporter gene expression was monitored by flow cytometry.

Caveolae expression was monitored by immunocytochemical co-staining with an antibody to caveolin-1.