Sentence examples for expression was made by from inspiring English sources

The induction of expression was made by the addition of 0.5% methanol at 24 h intervals.

Next, the analysis of gene expression was made by principal component analysis (PCA) to find a relationship with clinical pathological parameters.

Induction for protein expression was made by adding Isopropyl-1-thio-β-D-galactoside (IPTG) (Fermentas UAB, Vilnius, Lithuania) in a final concentration of 0.2 mM, and the growth was continued in 16°C for 16 h.

Digestion of CCKAR cDNA from the hypothalamus to determine allele-specific expression was made by comparing the sum of the 435- and 220-bp bands for the high-growth allele and the 655-bp band corresponding to the low-growth allele by a paired t-test.

Statistical comparisons between benign bladder and tumor bladder tissue expression were made by the Mann-Whitney U Test with SPSS 21.

Calculations to determine the relative level of gene expression were made by reference to the β-actin and SDHA in each sample, using the ΔCq method, and the results were depicted as arbitrary units.

Quantification of TNFSF4 expression was made relative to GAPDH by calculating the differences in C t (Δ C t ) and relative values determined by 2(−ΔΔ C t ) [ 8].

The pRK5Hr expression vector was made by inserting the XbaI SpeI fragment of the rat hr cDNA into the XbaI site of pRK5 as previously described [17].

The MUC1*1110 expression construct was made by cloning in frame the sequence corresponding to the C-terminal 145 amino acids of MUC1 beginning at GTINV.and ending at.AAAASANL after the nucleotide sequence for the signal peptide.

The deletion DISC1 expression construct was made by PCR-based mutagenesis protocol (24).

The pcDNA3.1 (Invitrogen, Paisley, United Kingdom) Wnt5a expression vector was made by cloning of the full-length PCR product of Wnt5a with PFU DNA polymerase (Invitrogen, Paisley, United Kingdom).