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The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction.
The monocytes and neutrophils protein expression was evaluated by flow cytometry.
Gene expression was evaluated by qRT-PCR.
Next, HIPK2 protein expression was evaluated by Western immunoblotting.
Gremlin expression was evaluated by Western blot and immunohistochemistry.
Chemokine receptors expression was evaluated by flow cytometry.
Gene expression was evaluated by reverse transcription quantitative polymerase chain reaction (RT-qPCR).
Cell surface EGFR expression was evaluated by flow cytometry performed as described.
Such expression was evaluated by western blotting in the same experimental conditions.
Gene expression was evaluated by real-time PCR as we previously described [32].
hCAREx8 expression was evaluated by immunocytochemistry (red fluorescence) using fluorescence microscopy and Western blot.
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