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Unigene expression was estimated by the FPKM method, and differentially expressed genes were identified by referenceto Audic [ 21].
Metalloproteinase (MMP) expression was estimated by immunohistochemical analysis.
hTERT expression was estimated by the copy number, and the U6 small nuclear RNA was used as an internal control.
After pcDNA-GFP-UTR was constructed, the GFP expression was estimated in A549 cells by the examination of fluorescence microscopy and flow cytometry.
The relational expression was estimated by statistical method on explanatory variable and objective variable about hydrolysis efficient by each enzyme.
Relative expression was estimated by counting the total number of ESTs for each FBX locus.
Breadth of gene expression was estimated as the number of organ and tissue sources of gene-specific ESTs.
γ-H2AX expression was estimated by anti-phospho-histone H2AX (Ser1∶100(1∶100) antibody and donkey anti-mouse IgG-FITC.
The degree of genetic and environmental influence on skeletal muscle ATP5O expression was estimated in young and elderly twins by means of intra-class correlations and biometric modeling.
Cytokine expression was estimated on a scale from 0 to +++; where +, ++ and +++ corresponded to 1 10, 10 20 and >20 cytokine expressing (positive) cells/section, respectively (Table 4).
Its protein expression was estimated to be only about 1% of that present in HL-60 cells and about 6% of that in PBMC.
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