MC-GP tumors with high endogenous COX-2 expression were established in immune competent C57BL/6 mice that were treated or not treated with celecoxib.
Their existence was first revealed in Drosophila melanogaster where homeotic gene (Hox) expression is established in early embryos by the transient Gap and Pair-Rule transcription factors and controlled by PcG and TrxG proteins during the rest of development [ 1- 3].
Depending on the area of positive immunoreactivity, a final overall score (high tumor B7-H3 or low tumor B7-H3 expression) was established as described in the methods section.
The tetracycline-inducible expression system was established in melanoma cells, and three fragments of the 5′-, centrand, and 3′-portion of the eIF-4A1 cDNA were subcloned in antisense and in sense orientation after a tetracycline inducible promoter.
Forced expression of AKR1C3 was established in human PCa PC-3 and LNCaP cell lines to recapitulate elevated AKR1C3 expression in cancer tissues and to study potential pathological activities of this enzyme in cancerous prostate.
Effective, significant FGF-2 gene transfer and expression via rAAV was established in the aspirates relative to the lacZ condition (from ~ 97 to 36 pg rhFGF-2/mg total proteins over an extended period of 21 days).
However, post-transcriptional suppression of gene expression by siRNA was established in oocytes and preimplantation embryos of mice [15] as well as in Xenopus embryos [16].
Therefore, no predictive ability of HER2 mRNA expression for paclitaxel was established in our study.
