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In the rCVB3-GFP infected HeLa cells and neonatal rat cardiac myocytes, GFP protein expression was documented by immunoblot and by fluorescent microscopy.

Morphologically, Capn3 expression was documented by immunohistochemical examination.

Morphologically, Capn3 expression was documented by immunohistochemical methods.

Using cell lysates isolated from those cells, an identical pattern of mPR α protein expression was documented by Western blot assays).

The two different populations, R1 and R2, in the MNL-suspension were gated and the composition of leukocytes subsets (CD14+, CD3+ and CD19+) and P2X7R expression was documented by specific antibodies analyzed by FACS.

RNAi knockdown of CD22ΔE12 expression was documented by RT-PCR analysis of RNA samples using the P7 (WT: 182 bp, Mutant: 63 bp) and P10 (213 bp, control) primer pairs after 48 h and Western blot analysis of whole cell lysates after 72 h, as described (Ma et al., 2012).

The expression of podocalyxin was documented by both immunofluorescent histology and RT-PCR analysis.

The expression of p16INK4A protein was documented by immunostaining using the procedure described elsewhere [13].

Expression of N1-ICD was documented by western blot in both CD24neg and CD24low+ sorted populations transduced with N1-ICD (Fig 8D).

Equal protein loading in each lane was documented by actin protein expression.

The differentiation state of the cells was documented by examining the expression of three markers of suprabasal keratinocytes: Keratin 10 [ 8], S100A7 (psoriasin) [ 9] and sPLA2 IIA [ 2].