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IL-10 expression was determined by qPCR.
Aortas eNOS expression was determined by Western blot.
The specific gene expression was determined by RT-PCR analysis.
PlexinD1 and CXCR4 expression was determined by western blotting and Cxcr4 expression using RT qPCR.
G9a expression was determined by western blotting with anti-G9a antibody.
(c) MHC and MYOG protein expression was determined by Western blot.
(A) JMJD2B expression was determined by qPCR (B) Immunostaining of JMJD2B (scale bar = 50 μm).
Gene expression was determined by real-time quantitative PCR, using TaqMan detection systems (Applied Biosciences).
Protein expression was determined by flow cytometry compared with an isotype-only control.
(f) MHC and MYOD1 protein expression was determined by Western blot.
HIF-1α expression was determined by western blot analysis.
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