Sentence examples for expression was determined as from inspiring English sources
S100A7/psoriasin and CXCL-8 mRNA expression was determined as well as CXCL-8 production using an ELISA (Biosource, Nivelles, Belgium) on cell culture supernatants.
Receptor-fluorescence expression was determined as fluorescence of the sample minus the fluorescence of cells expressing protein-Rluc alone.
On day 4, ALP expression was determined as previously described (Lee, S. S. et al. Adv Healthc Mater. 4, 2015).
μ opioid receptor expression was determined as described before [39].
β-actin expression was determined as loading controls.
E1A protein expression was determined as described earlier [11] using 50 µg of total lysates.
COX-1 and COX-2 mRNA expression were determined as described previously.
The cutoff values for abnormal expression were determined as follows: MDM2 > 30% [ 25]; p53 > 5% [ 26]; p16 < 5% [ 9].
In addition, multivariate analysis revealed that lymph node metastasis and MK expression were determined as independent prognostic factors.
Additionally, Samaco et al. [ 37] demonstrated differences in MeCP2 expression levels in autism, pervasive developmental disorder, Prader-Willi syndrome, and Angelman syndrome; by laser-scanning cytometry, the disturbances in MeCP2 expression were determined as due to differential transcriptional and post-transcriptional mechanisms.
Changes in gene expression were determined as log2 ratio of signal intensity values corresponding to transcripts present 4 hours after intake of olive oil with high phenol content, divided by the signal intensity values, which correspond to transcripts present 4 hours after consumption of low phenol content olive oil.
