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Quantification of phospho-AKT and phopsho-PRAS40 expression was determined.
HIF-1α expression was determined by western blot analysis.
Aortas eNOS expression was determined by Western blot.
The specific gene expression was determined by RT-PCR analysis.
Gene expression was determined using conventional and real-time PCR.
And p53 protein expression was determined via Western blot analysis.
PARP-1 and PAR expression was determined through Western blot.
CD36 expression was determined by quantitative PCR.
Receptor expression was determined by the mean fluorescence intensity (MFI).
Relative expression was determined using the delta Ct method.
HLA expression was determined in relation to EBV status.
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