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DDX21 expression was designated as 0 = no staining; 1 = weak; 2 = moderate; and 3 = strong.
Typically, the 3-5% of cells with the highest CD44 expression was designated as CD44high cell subset and the remainder of the population was designated as CD44low cell subset.
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HER2 expressing tumors without HR expression were designated as HR-/HER2+ (13 cases, 11.2%).
The two subpopulations with low and high GFP expression are designated as L and H subpopulations respectively.
Cells with reduced expression were designated as follows: A2780cisSN - snail knockdown, A2780cisSL - slug knockdown, A2780cisSN/SL - both snail and slug knocked down.
The difference in gene expression is designated as log2 and fold value (see Additional file 3: Table S3 for these two values).
With a focus on different combinations of these markers, we distinguished four distinct, non-overlapping classes: tumors positive for ER and/or PgR expression and negative for HER2 expression were designated as HR+/HER2- (57 cases, 49.1%).
This expression constructed was designated as Luc-HNF4G.
Finally, this expression vector was designated as the pRA2 vector (Fig. 1).
The mRNA expression below the median was designated as low expression and values above the median as high expression.
pMIR-REPORT Luciferase expression vector (Ambion) was digested using EcoR I and Hind III to delete the promoter of vector and the coding region of luciferase gene to produce pMIR-REPORT vector without mammalian expression promoter, which was designated as pMIR-REPORT-NoMp.
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