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Mineralization and osteocalcin expression was demonstrated by (immuno)histochemistry.
This construct was subsequently injected into rat eyes 24 hr prior to cauterization of the cornea and antisense vascular endothelial growth factor expression was demonstrated by in situ hybridization.
Suppressed P21 expression was demonstrated by Western blotting.
Reversibility of CD133 expression was demonstrated by transfering parental mitochondria to ρ0 cells resulting in stable trans-mitochondrial "cybrid" clones.
The HIVCONSV protein expression was demonstrated by immunofluorescence of transiently transfected or infected human embryonic kidney cells 293T using the mAb C-terminal tag (Fig. 2A D).
Lack of genomic episomal vector or transgene integration and expression was demonstrated by Southern blot, genomic PCR, and RT-PCR studies of plasmid backbone and transgene sequences (Figure 2D E).
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The validity and applicability of this expression were demonstrated by calculating the solvent activity in polymer solutions and analyzing the swelling behaviors for polymeric membranes.
The accuracy of this expression is demonstrated by both numerical simulations and experimental measurements of a model-scale five-story shear building, with damping introduced locally by a single eddy current damper.
Significant changes in gene expression were demonstrated by t test and linear regression [ 20].
In turn, an essential role of Fgf8 for the FoxG1 expression is demonstrated by the fact that neither Ras-dva1, nor Otx2 mRNA were able to rescue FoxG1 expression when these mRNA were co-injected with Fgf8 MO (0/35 and 0/32, respectively) (Fig. 6F; supplementary material Fig. S4J,K).
More recently, TM expression was demonstrated constitutively by some epithelial carcinomas, with decreased expression associated with metastases and high recurrence rates (Iino et al, 2004).
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