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Also, defensin PaDef protein expression was corroborated by flow cytometry.
Lack of toxin expression was corroborated by negative Vero cell assay results (7 ) and confirmed by using Western blot.
The prognostic value of WT1 gene expression was corroborated by later studies in patients with acute leukemia, nephroblastoma and breast cancer [ 13- 15, 29, 30].
The decrease in iNOS protein expression was corroborated by the reduced NO production by AS7 and AS12 cells, as determined by measuring the accumulation of nitrite following stimulation with TNF α, IFN γ and LPS for 24 h.
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The abundant immunohistochemical Fas and FasL expression were corroborated by western blot analysis.
This upregulation of gene expression is corroborated by an increase in Tcf19 protein expression with FoxM1 overexpression in human islet (Fig. 2 E ).
The observation that variant c.388A > G is associated with highest OATP1B1 protein expression is corroborated by our haplotype analysis since homozygous or compound heterozygous carriers of haplotypes *1b, *14, or *35, harboring c.388A > G but not c.521T > C, showed also the highest OATP1B1 protein levels.
Western blot confirmed exogenous TSP-1 expression, which was corroborated by antibody staining for Myc (Figure 4, lane 6).
Gene expression was analyzed by calculating reads per kilobase gene model per million reads (RPKM) and for selected genes in silico expression data was corroborated by qRT-PCR analysis.
Equivalent expression of each protein was corroborated by immunoblot using an anti-HA antibody (not shown).
Gene expression in each species was corroborated by the presence of a mosquito EST in the public database, as determined by blastn search, E value < 10-40.
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