Each plasmid was introduced into MH7A cells, and protein expression was confirmed by western blot analysis.
The correct knockout of Clock gene expression was confirmed by Western blot analysis and gene sequencing.
mRNA expression was confirmed by qPCR analysis using SYBR green method.
Functional TK-eGFP fusion protein expression was confirmed by Western blot analysis and ganciclovir uptake assay.
Toxin expression was confirmed in MaVipT31 hyphae and conidia using Western blotting.
Dependence on Sox9 expression was confirmed by electrophoretic mobility shift assays.
Moreover, antennal expression was confirmed for most genes included in the study.
Increased placental vascular endothelial growth factor expression was confirmed by immunohistologic examination.
Ad-CMV-HA-SSTR2 was constructed and expression was confirmed by in vitro experiments as previously described [18].
The influence of AA treatments on PSMA expression was confirmed by PSMA mRNA quantitation in CRPC cells.
In each case, CD44 expression was confirmed by central reviewing of tumour biopsies (Additional file 3: Figure S1).
