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Cells were then infected with LOX- or LOXL2-encoding vectors, two main LOX-family members expressed in HEC, and their expression was checked by immunoblotting.
As the first step, gene expression was checked upon using microarray, which is the main tool for global gene expression profiling of the response of an organism in a particular condition.
Protein expression was checked by SDS-PAGE.
Protein expression was checked by immunoblot as follows.
FoxP3 expression was checked again following cell sorting.
Protein expression was checked by Western blot analysis.
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Cases with intermediate level expression are checked by in situ hybridization, mainly by FISH, to identify amplifications in this subset of tumours.
The hepatic IGF-I and IGF-II gene expression were checked using real time PCR normalised against the β-actin gene and found to be maximum at the dietary inclusion level of 33%.
Furthermore, the effects on TRB3 protein expression were checked for some other individual amino acids.
FTL : Following one of our recent work, FTL and CD163 gene expression were checked (13).
E6 (HPV-16) and GAPDH expression were checked as described previously (Smeets et al, 2007).
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