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Cytokine-induced gene expression was calculated in comparison to unstimulated cells at the same time points.
Protein expression was calculated (in p.p.m).
Normalized mRNA expression was calculated in comparison to control samples relative to Gapdh.
Gene expression was calculated in spleen and liver in the same fashion.
Gene expression was calculated in reads per kilobase of exon per million mapped sequence reads (RPKM) [ 17].
SPARC mRNA expression was calculated in each sub-population of patients at diagnosis and after 3 months of IM treatment and compared with the correspondent subsets of HC.
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Error bars for gene expression were calculated in Excel using the standard deviation function.
Gene expression is calculated in Fragments Per Kilobase of Exon Per Million Fragments Mapped (FPKM).
Differential expression and corresponding p-values of differential expression were calculated in projected and residual space for a series of phenotypic variables.
Significant changes in gene expression were calculated in the form of Z ratios and/or Z test values ([ 25]), using Z score values in all calculations.
Expression is calculated in males for orphans of age classes 3 and 4. Expression level increases with age for conserved orphans (A ), while it decreases for lost orphans (B ).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com