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Measure of differential expression was calculated by setting the IVB expression levels as baseline and is expressed as fold difference.
The relative expression was calculated by the 2-∆∆Ct method.
Normalized fold expression was calculated by using 2−∆∆Ct method.
Relative gene expression was calculated by a comparative method using values normalized to the expression of an internal control gene.
Relative quantity of miR-214 and LHX6 mRNA expression was calculated by using the 2−ΔΔCT method.
Relative expression was calculated by comparison with a standard curve following normalization to expression of the housekeeping gene Gapdh, chosen as a control.
The relative gene expression was calculated by comparing cycle times for target PCR using the following equation: relative gene expression = 2 – (ΔCtsample − ΔCtcontrol).Values are normalized to housekeeping gene RPL-32 expression levels.
The fold expression was calculated by the 2−Δ ΔCt method as described by (Pfaffl 2001) (Eq. 1).
Differential expression was calculated by the Pfaffl method [111].
Expression was calculated by the ΔΔCt method as described previously [55].
Normalized expression was calculated by formula:{(EfficiencyhANT4) CT hANT4/ (EffiCTencyALG9} CT ALG9} using qGene software [31].
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