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Gene expression was assayed by microarray and 4157 differentially expressed genes (DEGs) were identified following ART using multivariate permutation tests.
Based on preliminary cytotoxicity results, gene expression was assayed by semi-quantitative RT-PCR as previously described [37].
Total RNA was isolated from either ClsA- or buffer-treated rice leaves; first-strand cDNA was synthesized, and gene expression was assayed by SYBR green-based real-time PCR.
AP expression was assayed by staining.
mRNA expression was assayed by RT-real-time PCR.
EGFP expression was assayed by fluorescence microscopy (Olympus DP70, BH2-RFL-T3).
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ALDH2 protein and mRNA expression were assayed by Western blot and real-time PCR, respectively.
Epidermal growth factor receptor expression was assayed as previously described by us (Olivier et al, 1990).
Protein expression level was assayed by western blot.
The mRNA expression pattern was assayed by RT PCR using panels of commercially available cDNA (Clontech, Palo Alto, CA, USA).
Expression of Smprx6 was assayed by quantitative real-time RT-PCR with β-actin as an internal control.
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