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The extinction of H4R protein expression was ascertained by FACS analysis after 24 h.
β2AR expression was ascertained using a highly quantitative 125I-cyanopindolol (125I-CYP) radioligand binding assay.
KIT expression was ascertained immunohistochemically and validated by immunoblotting.
GFP and YB-1-GFP fusion protein expression was ascertained by detection with monoclonal GFP antibody.
PPARγ protein expression was ascertained by immunocytochemical analysis (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-1984) 1 : 50.
20 ng, or the amounts specified in Figure 4D, of pCCF-Fam20A-Flag was co-transfected, when the effect of Fam20A ectopic expression was ascertained.
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For each gene model, the growth stage and the plant organ which showed maximal expression were ascertained and maximal expression profiles for growth stage and plant organs relative to the Arabidopsis gene models were constructed.
Chemerin and ChemR23 expression in the RA synovium was ascertained by immunohistochemistry and Western blot analysis.
Impact of HOTAIR expression on ovarian cancer survival was ascertained using log rank test and Kaplan Meier curves.
Regarding survival analysis, no impact of Hiwi mRNA expression on tumour-related death was ascertained for all PDAC patients.
To address this question the skeletal muscle specificity (SPi: see Materials and Methods: Data Input to the Virtual Muscle) of the expression of each of the genes was ascertained by interrogating the SymAtlas mouse GNF1M dataset which contains a tissue survey of mouse gene expression (see Additional File 1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com