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CKI-ε and p32FoxO3 expression was analyzed by Western blotting.
SFAR4 transcript expression was analyzed by RT-PCR.
Hence, PTH mRNA expression was analyzed by RT-PCR.
Protein expression was analyzed by Western blot as described below.
Quantification of the relative gene expression was analyzed by the 2−ΔΔCt method.
mRNA expression was analyzed by a RT-PCR as previously described [21, 25, 26].
Gene expression was analyzed by real-time PCR, and normalized using GAPDH expression level.
Gene expression was analyzed by semi-quantitative PCR using GAPDH as a standard.
One-week-old seedlings were treated with BR, and AtBS14b expression was analyzed by qRT-PCR.
Further, gene expression was analyzed by microarray studies on MCF-7 cell lines.
Protein expression was analyzed by 2D electrophoresis, gel densitometry and mass spectrometry.
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