The fluorescence intensity of α-SMA expression was analysed using ImageJ software.
The relative gene expression was analysed by comparative 2−ΔΔCt method.
The relative gene expression was analysed by comparative 2−ΔΔct method.
Expression was analysed separately in the tumour centre and advancing front; and prognostic capability determined using Kaplan Meier survival analysis.
To determine if Trex1 could control the abundance of cytoplasmic DNA in TSA cells treated with different radiation doses, its expression was analysed.
Transgene expression was analysed by western blotting with 2 μg anti-c-MYC (Sigma, C3956) antibody or HERK1 antibody as control.
Integrin expression was analysed by qPCR, which showed the extent of spatial and temporal regulation achieved by the nanopores.
Dll4 expression was analysed by RQ-PCR and flowcytometry.
Surface receptors expression was analysed by FACS (FACSCanto, Becton-Dickinson).
hSAA expression was analysed using real-time PCR analysis.
Gene expression was analysed using low-density arrays.
